This folder contains the codes to analize the expeirments of a single molecule pulling experiment discussed in the text.
How to use the Single molecule mechanics section:
Run work_distributions in folder programs to obtain (A) Several pulling trajectories of the hairpin. In red are plotted the unfolding cycles and in blue the folding ones. The black dots indicate the initial ( L0 ; fmin) and final L1 ; fmax) points considering for computing the work, WFU. (B) Probability density distributions of the work, computed as in equation (6). The point at which the probabilities cross is the equilibrium difference of free energy of the hairpin. The red and blue lines shown are the interpolations of the bars.
The files contained in data '' folder u,f for the unfolding(stretching) and folding(releasing) trajectories. They contain 4 columns: $1=counter $2=trap distance(nm) $3=time (s) $4=force(pN) The files have been sorted in order to have the trap distance order toward increasing values.
The program will run the correspondent analysis and save the output in folder results, this is useful if you want to use another program for doing th plot.
This repository includes the analysis and plot codes for the the article Optical Tweezers: A comprehensive Tutorial from Calibration to Applications by Jan Gieseler, Juan Ruben Gomez-Solano, Alessandro Magazù, Isaac Pérez Castillo, Laura Pérez García, Martha Gironella-Torrent, Xavier Viader-Godoy, Felix Ritort, Giuseppe Pesce, Alejandro V. Arzola, Karen Volke-Sepulveda and Giovanni Volpe.