Description of feature
Feature integration in read_metadata(img_path, image_type="czi", parse_metadata=True)
- extract scene information from czi file. (e.g. 'scene': [0,1,2,3] or 'scene': (0, 4))
- extract exposure time and intensity (e.g. 'exposure time': [50, 100, ...] (in ms); 'intensity': [50,100,100, ...] (in %))
Optional:
3. If possible also extract dimensions of czi or each scene.
4. Extract dye information for better knowledge -> 'Fluor' in metadata
Example for 3.:
'dimensions': [{'X': (0, 2236), 'Y': (0, 2236), 'Z': (0, 1), 'C': (0, 6), 'M': (0, 42), 'S': (0, 1), 'H': (0, 1)}]
'shape_info': HSCZMYX
or
'shape_info': HSCZMYX
'shape': (1, 1, 6, 1, 42, 2236, 2236)
Example for 4.:
'dye': ['TL Brightfield', 'Alexa Fluor 633', 'Alexa Fluor 555', 'Alexa Fluor 488', 'DAPI', 'Cy7']
e.g. metadata (extracted information in bold)
'Dimensions': {'Channels': {'Channel': [
{'@id': 'Channel:1',
'@name': 'AF633',
'ExcitationWavelength': '631',
'EmissionWavelength': '647',
'DyeId': 'McNamara-Boswell-0055',
'DyeDatabaseId': '66071726-cbd4-4c41-b371-0a6eee4ae9c5',
'Color': '#FFFF00FF',
'Fluor': 'Alexa Fluor 633',
'ExposureTime': '700000000',
'IlluminationType': 'Epifluorescence',
'ContrastMethod': 'Fluorescence',
'PixelType': 'Gray16',
'ComponentBitCount': '16',
'AcquisitionMode': 'WideField',
'IlluminationWavelength': {'SinglePeak': '630', 'Ranges': '615-648'},
'DetectorSettings': {'Binning': '1,1',
'HasFocusRoi': 'true',
'Detector': {'@id': 'Detector:Hamamatsu Orca Flash'}},
'LightSourcesSettings': {'LightSourceSettings': {'Intensity': '100.00 %',
'LightSource': {'@id': 'LightSource:2'}}},
'FilterSetRef': {'@id': 'FilterSet:2'}},
Description of feature
Feature integration in read_metadata(img_path, image_type="czi", parse_metadata=True)
Optional:
3. If possible also extract dimensions of czi or each scene.
4. Extract dye information for better knowledge -> 'Fluor' in metadata
Example for 3.:
'dimensions': [{'X': (0, 2236), 'Y': (0, 2236), 'Z': (0, 1), 'C': (0, 6), 'M': (0, 42), 'S': (0, 1), 'H': (0, 1)}]
'shape_info': HSCZMYX
or
'shape_info': HSCZMYX
'shape': (1, 1, 6, 1, 42, 2236, 2236)
Example for 4.:
'dye': ['TL Brightfield', 'Alexa Fluor 633', 'Alexa Fluor 555', 'Alexa Fluor 488', 'DAPI', 'Cy7']
e.g. metadata (extracted information in bold)
'Dimensions': {'Channels': {'Channel': [
{'@id': 'Channel:1',
'@name': 'AF633',
'ExcitationWavelength': '631',
'EmissionWavelength': '647',
'DyeId': 'McNamara-Boswell-0055',
'DyeDatabaseId': '66071726-cbd4-4c41-b371-0a6eee4ae9c5',
'Color': '#FFFF00FF',
'Fluor': 'Alexa Fluor 633',
'ExposureTime': '700000000',
'IlluminationType': 'Epifluorescence',
'ContrastMethod': 'Fluorescence',
'PixelType': 'Gray16',
'ComponentBitCount': '16',
'AcquisitionMode': 'WideField',
'IlluminationWavelength': {'SinglePeak': '630', 'Ranges': '615-648'},
'DetectorSettings': {'Binning': '1,1',
'HasFocusRoi': 'true',
'Detector': {'@id': 'Detector:Hamamatsu Orca Flash'}},
'LightSourcesSettings': {'LightSourceSettings': {'Intensity': '100.00 %',
'LightSource': {'@id': 'LightSource:2'}}},
'FilterSetRef': {'@id': 'FilterSet:2'}},